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90
SeraCare Life Sciences dna detector genomic southern blotting kit
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Dna Detector Genomic Southern Blotting Kit, supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+advanced+science/dna+detector+genomic+southern+blotting+kit/pmc00154050-107-29-35
Average 90 stars, based on 1 article reviews
dna detector genomic southern blotting kit - by Bioz Stars, 2026-09
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Xi'an Tianlong Science whole blood genome dna puri cation kit
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Whole Blood Genome Dna Puri Cation Kit, supplied by Xi'an Tianlong Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+advanced+science/whole+blood+genome+dna+puri+cation+kit/ppr0136380-36-10-16
Average 90 stars, based on 1 article reviews
whole blood genome dna puri cation kit - by Bioz Stars, 2026-09
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Tepnel Life Sciences PLC nucleon genomic dna extraction kit
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Nucleon Genomic Dna Extraction Kit, supplied by Tepnel Life Sciences PLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
nucleon genomic dna extraction kit - by Bioz Stars, 2026-09
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Corning Life Sciences the genomic dna miniprep kit
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
The Genomic Dna Miniprep Kit, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+advanced+science/genomic+dna+miniprep+kit/pm34016129-121-2-5
Average 90 stars, based on 1 article reviews
the genomic dna miniprep kit - by Bioz Stars, 2026-09
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Corning Life Sciences genomic 119 dna purification kit
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Genomic 119 Dna Purification Kit, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+advanced+science/genomic+dna+purification+kit/10__1042_slash_bsr20180498-54-10-14
Average 90 stars, based on 1 article reviews
genomic 119 dna purification kit - by Bioz Stars, 2026-09
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Corning Life Sciences multisource genomic dna miniprep kit
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Multisource Genomic Dna Miniprep Kit, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences axy-prep genomic dna miniprep kit
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Axy Prep Genomic Dna Miniprep Kit, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axy-prep genomic dna miniprep kit - by Bioz Stars, 2026-09
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AllBio Science Inc allpure micro genomic dna extraction kit
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Allpure Micro Genomic Dna Extraction Kit, supplied by AllBio Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+advanced+science/allpure+genomic+dna+extraction+kit/pm35033061-61-23-32
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allpure micro genomic dna extraction kit - by Bioz Stars, 2026-09
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Advanced Biotechnologies Inc genomic dna from chlamydia trachomatis strain 434 lgv ii
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Genomic Dna From Chlamydia Trachomatis Strain 434 Lgv Ii, supplied by Advanced Biotechnologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genomic dna from chlamydia trachomatis strain 434 lgv ii - by Bioz Stars, 2026-09
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Advanced Biotechnologies Inc quantified genomic dna from chlamydia trachomatis ( c. trachomatis ) strain 434 lgv ii
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Quantified Genomic Dna From Chlamydia Trachomatis ( C. Trachomatis ) Strain 434 Lgv Ii, supplied by Advanced Biotechnologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantified genomic dna from chlamydia trachomatis ( c. trachomatis ) strain 434 lgv ii - by Bioz Stars, 2026-09
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Advanced Biotechnologies Inc purified chlamydia pneumoniae (ar-139) genomic dna
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Purified Chlamydia Pneumoniae (Ar 139) Genomic Dna, supplied by Advanced Biotechnologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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purified chlamydia pneumoniae (ar-139) genomic dna - by Bioz Stars, 2026-09
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Advanced Biotechnologies Inc hhv-6 control dna
Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer <t>DNA</t> biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic <t>Southern</t> <t>blotting</t> kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.
Hhv 6 Control Dna, supplied by Advanced Biotechnologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomic+dna+advanced+science/hhv+6+genomic+dna/10__5897_slash_jahr2016__0382-79-9-13
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hhv-6 control dna - by Bioz Stars, 2026-09
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Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer DNA biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic Southern blotting kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.

Journal:

Article Title: Disruption of Gammaherpesvirus 68 Gene 50 Demonstrates that Rta Is Essential for Virus Replication

doi: 10.1128/JVI.77.10.5731-5739.2003

Figure Lengend Snippet: Genomic structure of gene 50 recombinant viruses. (A) Southern blot analysis. (Left) wt, G50.Stop.[Frt-Kan-Frt], and G50.Stop.Frt BAC viral genomes were purified from E. coli DH10B cells, digested with SpeI, electrophoresed, blotted, and hybridized with a biotin-labeled gene 50 probe (bp 66642 to 69462). The biotin-labeled gene 50 probe was generated by using the Detector random primer DNA biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic Southern blotting kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with XbaI, electrophoresed, blotted, and hybridized with a 32P-labeled gene 44 probe (bp 61444 to 62183). On both panels, the fragment sizes of the molecular size markers are shown to the left of each blot (1-kb DNA ladder for the left panel, lambda DNA-BstEII digest for the right panel; New England Biolabs). To the right of each blot are shown the predicted sizes of the viral DNA fragments detected by the respective probes in each blot. (B) Nucleotide sequence of the region containing the mutation in the G50.Stop.Frt BAC. The genome coordinates are to the right of the nucleotide sequence. The nucleotide sequence shown in lowercase letters denotes the gene 50 intron of the spliced gene 50 transcript, and the sequence in uppercase letters denotes the second gene 50 exon of the spliced gene 50 transcript. The splice acceptor site is denoted with an arrowhead. The ORF 50 ATG is boxed. The mutation in G50.Stop.Frt BAC is depicted in boldface, the introduced TAG stop codon is boxed, the FRT site is underlined, and the XbaI site within the FRT site is boxed and italicized.

Article Snippet: The biotin-labeled gene 50 probe was generated by using the Detector random primer DNA biotinylation kit (Kirkegaard & Perry Laboratories), and the Southern blot was developed by using the DNA Detector genomic Southern blotting kit (Kirkegaard & Perry Laboratories) according to the manufacturer's instructions. (Right) wt γHV68 or wt BAC and G50.Stop.Frt BAC (G50 KO in the text) viral genomes were purified from virions isolated from NIH 3T12 and gene 50-expressing stable cell line 14.29, respectively, digested with Xba I, electrophoresed, blotted, and hybridized with a 32 P-labeled gene 44 probe (bp 61444 to 62183).

Techniques: Recombinant, Southern Blot, Purification, Labeling, Generated, Isolation, Expressing, Stable Transfection, Lambda DNA Preparation, Sequencing, Mutagenesis